The experiments took place by the leaf-dipping technique. Determination of lethal concentrationsĪbout six concentrations of methomyl were prepared by using dechlorinated tap water on the basis of weight/volume ( Osman et al., 2011, Osman and Mohamed, 1991) as follows (0.07, 0.14, 0.28, 0.56, 1.12, and 1.3 g/L). Also, the effect of the above-mentioned sub-lethal concentrations on the histological structure of snails, digestive gland, hermaphrodite gland, foot, and mantle was studied.Ģ.3.1. This study was planned to assay the effect of the methomyl (Copter 90%) pesticide with their sub-lethal concentrations LC 20 (0.075 g/L) and LC 40 (0.180 g/L) on some biochemical activities alkaline phosphatase (ALP), alanine amino transaminase (ALT), Aspartate amino transaminase (AST) and total protein (TP) at three lethality exposure periods of Monacha cartusina land snail. vestalis were exposed to both methiocarb and Chlorpyrifos ( Sharaf et al., 2015). Also, alterations in the biochemical parameters alkaline phosphatase (ALP), alanine amino transaminase (ALT), Aspartate amino transaminase (AST), and total protein (TP) meanwhile, some histopathological alterations will be observed in many organs in the land snail H. The effects of pesticides on land snails will recognize physiologically by assaying the biochemical and histological changes ( Abo Baker, 2011, Hamed et al., 2007, Khalil, 2016). It is used as an abroad-spectrum insecticide in field crops, ornamental plants, vegetables, cotton, and fruit fields ( Mortensen and Serex, 2014). Also, methomyl is the most pesticide used in the form of bait technic for terrestrial gastropods control ( Abdallah et al., 2015). Methomyl (Copter 90%) is a systemic chemical compound of monomethylcarbamate insecticide used for pest management in some field crops ( Khalil, 2016, Khidr, 2019). The control of snails with chemical compounds was widely distributed and more effective. They are found in vegetable and field, fruit crops, orchards, ornamental and medical plants ( Abou Senna et al., 2016, Heikal, 2015, Kadry et al., 2018, Rady, 2019, Shahawy, 2018, Shetaia et al., 2009). Likewise, Monacha cartusiana (Müller, 1774), Mollusca, Gastropoda, Pulmonata, Stylomatophora, Helicoidae, Monacheae were widely distributed in the Egyptian Governorates. ![]() Nowadays, land snails are considered the most injurious pest in stylommatophora in Egypt ( Ali and Robinson, 2020, Heiba et al., 2002). Moreover, mantle shows rapture in epidermis layer, deformed in muscle fiber layer, and vacuolization and necrosis take place in mucus gland. Likewise, deformation in the muscle fiber layer of the foot in the land snail distorts the epidermis and mucus gland suffering from necrosis. The Oocyte and sperm show degenerated with deformation in the connective tissue of the hermaphrodite gland. Degeneration, rupture, and vacuolization for digestive cells have been shown furthermore, hemolytic infiltration in connective tissue will be recognized for the digestive gland. Both concentrations of Copter (0.0.75 g/L and 0.180 g/L) have shown histological changes for land snail tissues after 96 h of exposure digestive gland, hermaphrodite gland, foot, and mantle. In contrast, a total protein (TP) activity decreased at exposure for two concentrations at all lethality periods. This study cleared that both the used concentrations (of Copter) caused a significant increase for activities of three enzymes: alkaline phosphatase (ALP), alanine amino transaminase (ALT), and Aspartate amino transaminase (AST) after 24, 48, and 72 h from exposure starting. ![]() Land snails belong to the class Gastropoda and Phylum Mollusca. This manuscript was conducted to spotlight the toxic effect of two sub-lethal concentrations of Methomyl (Copter) LC 20 (0.075 g/L) and LC 40 (0.180 g/L) on some biochemical parameters and histological alterations for land snail Monacha Cartusiana (Muller, 1774).
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